Crocin ameliorated skin tissue inflammation in atopic dermatitis in mice




β-catenin, Interleukin -1β, Nuclear factor κB, Tumor necrosis factor


Background: Atopic dermatitis (AD) is considered a chronic recurrent inflammatory skin disease. In addition, crocin is the major carotenoid compound found in Gardenia jasminoides. It is previously proved to produce anti-inflammatory actions. Therefore, we conducted this research to investigate the therapeutic effects of crocin on a mice model of AD.

Methods: Mice were investigated for the number of scratches and dermatitis score. Skin was isolated and used for measurements of gene and protein expression of β-catenin, NFκB, TNF-α and IL-1β.

Results: Authors found that crocin significantly reduced the number of scratches, ear thickness and dermatitis score. In addition, crocin ameliorated AD-induced elevation in the expression of β-catenin, NFκB, TNF-α and IL-1β.

Conclusions: Crocin ameliorated DNCB-induced AD in mice via blockage of β-catenin with subsequent reduction in inflammatory pathway.



Torres T, Ferreira EO, Goncalo M, Mendes-Bastos P, Selores M, Filipe P. Update on Atopic Dermatitis. Acta medica portuguesa. 2019;32:606-13.

Weidinger S, Beck LA, Bieber T, Kabashima K, Irvine AD. Atopic dermatitis. Nature Revi Dis Prim. 2018;4:1.

Silverberg JI, Gelfand JM, Margolis DJ, Boguniewicz M, Fonacier L, Grayson MH, et al. Health Utility Scores of Atopic Dermatitis in US Adults. J Aller Clin Immunol Pract. 2019;7:1246-52.

Boguniewicz M, Alexis AF, Beck LA, Block J, Eichenfield LF, Fonacier L, et al. Expert perspectives on management of moderate-to-severe atopic dermatitis: a multidisciplinary consensus addressing current and emerging therapies. J Aller Clin Immunol: Pract. 2017;5(6):1519-31.

Jung WS, Chae YS, Kim DY, Seo SW, Park HJ, Bae GS, et al. Gardenia jasminoides protects against cerulein-induced acute pancreatitis. World J Gastroenterol: WJG. 2008;14:6188-94.

Alyoussef A. Arjunolic acid protects against DNCB-induced atopic dermatitis-like symptoms in mice by restoring a normal cytokine balance. Eur Cytok Network. 2015;26:38-45.

Sung YY, Lee AY, Kim HK. The Gardenia jasminoides extract and its constituent, geniposide, elicit anti-allergic effects on atopic dermatitis by inhibiting histamine in vitro and in vivo. J Ethnopharmacol. 2014;156:33-40.

Park JH, Lee KY, Park B, Yoon J. Suppression of Th2 chemokines by crocin via blocking of ERK-MAPK/NF-kappaB/STAT1 signalling pathways in TNF-alpha/IFN-gamma-stimulated human epidermal keratinocytes. Exper Dermatol. 2015;24:634-6.

Moy AP, Murali M, Kroshinsky D, Horn TD, Nazarian RM. T-helper immune phenotype may underlie 'paradoxical' tumour necrosis factor-alpha inhibitor therapy-related psoriasiform dermatitis. Clin Exper Dermatol. 2018;43:19-26.

Alshevskaya AA, Lopatnikova JA, Krugleeva OL, Nepomnyschih VM, Lukinov VL, Karaulov AV, et al. Expression density of receptors to IL-1beta in atopic dermatitis. Mol Immunol. 2016;75:92-100.

Liu W, Sun Y, Cheng Z, Guo Y, Liu P, Wen Y. Crocin exerts anti-inflammatory and anti-arthritic effects on type II collagen-induced arthritis in rats. Pharma Biol. 2018;56:209-16.

Mohammadzadeh L, Hosseinzadeh H, Abnous K, Razavi BM. Neuroprotective potential of crocin against malathion-induced motor deficit and neurochemical alterations in rats. Envir Sci Pollut Res Inter. 2018;25:4904-14.

Elsherbiny NM, Salama MF, Said E, El-Sherbiny M, Al-Gayyar MM. Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-regulation of inflammatory and apoptic pathways. Chemico-biol Inter. 2016;247:39-48.






Original Research Articles