Comparative efficacy of topical hair growth serums on hair density and shedding: a double-blinded and randomized clinical trial
DOI:
https://doi.org/10.18203/issn.2455-4529.IntJResDermatol20261929Keywords:
Alopecia, Bhringaraj, Hair fall, Hair growth, Hair loss, Phototrichogram, Clinical dermatologyAbstract
Background: This clinical evaluation conducted with the objective of comparing the efficacy of two topical hair growth serum formulations on healthy Indian adults (men and women) suffering from hair loss, by randomized, double blinded controlled study.
Methods: Two topical formulations (product A - Indulekha Bringha scalp serum and product B – 3% Redensyl in serum vehicle) were evaluated over 3 months, with a 2-week regression phase. The study included a wash out period, following which subjects were randomized in one of the treatment groups. Subjects visited the study centre once every month for their follow-up assessments during treatment period. Efficacy endpoints included anagen hair density, A:T ratio, hair pull test, hair fall assessments, scalp barrier function, and hair thickness evaluation.
Results: The Indulekha Bringha scalp serum (product A) was found to be significantly efficacious in reducing hair shedding, improving hair density in all the subjects in comparison to baseline and was significantly superior to product B in the studied hair growth parameters.
Conclusions: Both test products demonstrated statistically significant improvements from baseline across all evaluated hair growth parameters. However, Indulekha Bringha scalp serum (product A) showed significantly superior efficacy compared to the product B in improving Anagen density, reducing hair fall.
References
Owecka B, Tomaszewska A, Dobrzeniecki K, Owecki M. The hormonal background of hair loss in non-scarring alopecias. Biomedicines. 2024;12(3):513. DOI: https://doi.org/10.3390/biomedicines12030513
Sankhwar S, Khan AI. Systematic review: causes and treatments of hair loss. J Dermatol Case Rep. 2025;18:118-28.
Varman PM, Paul CMP, Rajan P, Preethi R, Premkumar, Priya R, et al. Study on hair fall with hair related problems among males of age 18-50 years: study on Chennai-based population. J Clin Diagn Res. 2018;12(10):LC09-12. DOI: https://doi.org/10.7860/JCDR/2018/36050.12175
Gokce N, Basgoz N, Kenanoglu S, Akalin H, Ozkul Y, Ergoren MC, et al. An overview of the genetic aspects of hair loss and its connection with nutrition. J Prev Med Hyg. 2022;63(2):E228-38.
Alanazi AS, Alsalhi WA, Alghuyaythat WK, Almutairi AN, Almazrou MA, Alabdulminaim JA, et al. Stress-related hair loss among the general population in Al Majma'ah, Saudi Arabia: a cross-sectional study. Cureus. 2023;15(10):e46517. DOI: https://doi.org/10.7759/cureus.46517
Sinclair R. Hair shedding in women: how much is too much? Br J Dermatol. 2025;173(3):846-8. DOI: https://doi.org/10.1111/bjd.13873
Adav SS, Ng KW. Recent omics advances in hair aging biology and hair biomarkers analysis. Ageing Res Rev. 2023;91:102041. DOI: https://doi.org/10.1016/j.arr.2023.102041
O'Connor K, Goldberg LJ. Nutrition and hair. Clin Dermatol. 2021;39(5):809-18. DOI: https://doi.org/10.1016/j.clindermatol.2021.05.008
Trüeb RM. Oxidative stress in ageing of hair. Int J Trichology. 2009;1(1):6-14. DOI: https://doi.org/10.4103/0974-7753.51923
Santos Z, Avci P, Hamblin MR. Drug discovery for alopecia: gone today, hair tomorrow. Expert Opin Drug Discov. 2015;10(3):269-92. DOI: https://doi.org/10.1517/17460441.2015.1009892
Cash TF, Price VH, Savin RC. Psychological effects of androgenetic alopecia on women: comparisons with balding men and with female control subjects. J Am Acad Dermatol. 1993;29(4):568-75. DOI: https://doi.org/10.1016/0190-9622(93)70223-G
Hunt N, McHale S. The psychological impact of alopecia. BMJ. 2005;331(7522):951-3. DOI: https://doi.org/10.1136/bmj.331.7522.951
Asghar F, Shamim N, Farooque U, Sheikh H, Aqeel R. Telogen effluvium: a review of the literature. Cureus. 2020;12(5):e8320. DOI: https://doi.org/10.7759/cureus.8320
Kaliyadan F, Nambiar A, Vijayaraghavan S. Androgenetic alopecia: an update. Indian J Dermatol Venereol Leprol. 2013;79(5):613-25. DOI: https://doi.org/10.4103/0378-6323.116730
Sinclair R, Torkamani N, Jones L. Androgenetic alopecia: new insights into the pathogenesis and mechanism of hair loss. F1000Research. 2015;4:585. DOI: https://doi.org/10.12688/f1000research.6401.1
Abeck F, Hansen-Abeck I, Kött J, Garrahy E, Schneider SW, von Büren J. Direct-to-consumer teledermatology for male androgenetic alopecia: narrative review. JMIR Dermatol. 2025;8:e72704. DOI: https://doi.org/10.2196/72704
Rezan Ekmekci T, Koslu A. Phototrichogram findings in women with androgenetic alopecia. Skin Res Technol. 2006;12(4):309-12. DOI: https://doi.org/10.1111/j.0909-752X.2006.00196.x
Janaki CS, Sachdev M, Ritambhara. A single-arm exploratory pilot study to evaluate the efficacy of a multi-component water-based herbal supplement in hair growth, density, follicle strength and hair fall in healthy male and female subjects. IP Indian J Clin Exp Dermatol. 2021;7(3):249-55. DOI: https://doi.org/10.18231/j.ijced.2021.047
Dhurat R, Saraogi P. Hair evaluation methods: merits and demerits. Int J Trichol. 2009;1(2):108-19. DOI: https://doi.org/10.4103/0974-7753.58553
Rogers GE. Hair follicle differentiation and regulation. Int J Dev Biol. 2004;48(2-3):163-70. DOI: https://doi.org/10.1387/ijdb.15272381
Hsu JM, Anthony WL. Impairment of cystine-35S incorporation into skin protein by zinc-deficient rats. J Nutr. 1971;101(4):445-52. DOI: https://doi.org/10.1093/jn/101.4.445
Bikash C. Topical alternatives for hair loss: beyond the conventional. Int J Trichology. 2025;17(1):13-9. DOI: https://doi.org/10.4103/ijt.ijt_8_23
Katoulis AC, Liakou AI, Koumaki D, Vakirlis E, Tsantes AG, Mortaki D, et al. A randomized, single-blinded, vehicle-controlled study of a topical active blend in the treatment of androgenetic alopecia. Dermatol Ther. 2020;33(4):e13734. DOI: https://doi.org/10.1111/dth.13734
Vashishtha NM, Mohar PM. Review on Nilibhringadi Taila: an effective keshya medication. AYUSHDHARA. 2022;9(6):111-4. DOI: https://doi.org/10.47070/ayushdhara.v9i6.1103
Begum S, Lee MR, Gu LJ, Hossain J, Sung CK. Exogenous stimulation with Eclipta alba promotes hair matrix keratinocyte proliferation and downregulates TGF-β1 expression in nude mice. Int J Mol Med. 2015;35:496-502. DOI: https://doi.org/10.3892/ijmm.2014.2022
Kumari I, Kaurav H, Chaudhary GI. Eclipta alba (bhringraj): a promising hepatoprotective and hair growth stimulating herb. Asian J Pharm Clin Res. 2021;14(7):16-23. DOI: https://doi.org/10.22159/ajpcr.2021.v14i7.41569
Du F, Li J, Zhang S, Zeng X, Nie J, Li Z. Oxidative stress in hair follicle development and hair growth: signalling pathways, intervening mechanisms and potential of natural antioxidants. J Cell Mol Med. 2024;28:e18486. DOI: https://doi.org/10.1111/jcmm.18486
Bi D, Hua S, Hu Y, Liu J, Guo S. The pathogenesis of oxidative stress in androgenetic alopecia. Dermatol Ther. 2025;2025:4548932. DOI: https://doi.org/10.1155/dth/4548932
Jang SH, Kim MJ, Wee JH, Kim JT, Choi WH. Effects of Amla (Phyllanthus emblica L.) extract on hair growth promoting. Korean Soc Biotechnol Bioeng J. 2018;33:299-305. DOI: https://doi.org/10.7841/ksbbj.2018.33.4.299
Randall VA, Botchkareva NV. The biology of hair growth. In: Cosmetics applications of laser & light-based systems. 2009:3-5. DOI: https://doi.org/10.1016/B978-0-8155-1572-2.50006-3
Sakita S, Ohtani O, Nakatani T, Morohashi M. Three-dimensional microvasculature of the hair follicle. J Dermatol Sci. 1994;7:S1-4. DOI: https://doi.org/10.1016/0923-1811(94)90028-0
Yano K, Brown LF, Detmar M. Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest. 2001;107(4):409-17. DOI: https://doi.org/10.1172/JCI11317
Lachgar S, Moukadiri H, Jonca F, Charveron M, Bouhaddioui N, Gall Y, et al. Vascular endothelial growth factor is an autocrine growth factor for hair dermal papilla cells. J Invest Dermatol. 1996;106(1):17-23. DOI: https://doi.org/10.1111/1523-1747.ep12326964
Madhavi MSL, Shukla RK, Gudulkar S, Jadhav AM, Mathur S, Punyani S. Hair growth efficacy and safety assessment of an ayurvedic leave-on treatment: a clinical trial. Int J Res Dermatol. 2025;11:518-26. DOI: https://doi.org/10.18203/issn.2455-4529.IntJResDermatol20253397
Kwon OS, Han JH, Yoo HG, Chung JH, Cho KH, Eun HC, et al. Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate (EGCG). Phytomedicine. 2007;14(7-8):551-5. DOI: https://doi.org/10.1016/j.phymed.2006.09.009
Nieto G, Ros G, Castillo J. Antioxidant and antimicrobial properties of rosemary (Rosmarinus officinalis, L.): a review. Medicines. 2018;5(3):98. DOI: https://doi.org/10.3390/medicines5030098
Ningappa MB, Ramadas D, Nanjegowda D, Balasubramanian K, Chahal K, Patil S, et al. Cytoprotective properties of antioxidant protein from curry leaves (Murraya koenigii L.) against oxidative stress induced damage in human erythrocytes. Med Chem. 2016;6(2):2161-444. DOI: https://doi.org/10.4172/2161-0444.1000328
Patel M, Tuli N, Patel N, Merja A, Patel MN. A clinical evaluation of the safety, efficacy, and tolerability of the Soulflower Rosemary Redensyl Hair Growth Serum, Tetragain™, in healthy female subjects for the treatment of alopecia: promoting hair growth and reducing gray hair. Cureus. 2025;17(1):e77066. DOI: https://doi.org/10.7759/cureus.77066
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