A pilot, exploratory study to demonstrate the efficacy of a novel adjuvant herbal liquid supplement for skin rejuvenation and anti-aging


  • C. S. Janaki Department of Anatomy, Bhaarath Medical College, Chennai, Tamil Nadu, India http://orcid.org/0000-0002-2780-8822
  • Mukta Sachdev MS Clinical Research Pvt Ltd, Bangalore, Karnataka, India
  • Ritambhara K. R. MS Clinical Research Pvt Ltd, Bangalore, Karnataka, India




Diabliss herbal skin water supplement, Anti-aging, Wrinkles, Hydration, Pigmentation


Background: Aging of skin has both intrinsic and extrinsic causative factors. Impact of oral consumption of a novel multi-component herbal skin water supplement (HSWS) by Diabliss was investigated in a pilot exploratory study.

Methods: A 90-day open label clinical study among 40 healthy male and female subjects aged 35-50 years, presenting with signs of aging including wrinkles and pigmentation related skin concerns. The study assessments included dermatological, instrumental and imaging evaluation to investigate the impact of the Diabliss HSWS in providing improvements in various signs of aging.

Results: The Diabliss HSWS was able to show significant improvement in skin elasticity parameters by Cutometer® with improvements that ranged between 23-54% for the six elasticity parameters, skin hydration improvements as measured by Corneometer® in forehead (30%) and cheek (34%), wrinkles reduction reductions by Antera® in forehead (25%) and crows feet area (20%), texture improvements in forehead (25%) and crows feet area (20%), reduction L* value on the localized pigmentation/ spot of 6%. The subject assessments also reported improvement in the skin and general health. There were no product related AE/SAE and the product was found to be safe and well tolerated.

Conclusions: In conclusion, this water based oral nutraceutical is a novel delivery system for a skin rejuvenation adjuvant therapy which is cosmetically and systemically acceptable and tolerable for patients.

Author Biographies

C. S. Janaki, Department of Anatomy, Bhaarath Medical College, Chennai, Tamil Nadu, India

Associate Professor, Department of Anatomy

Mukta Sachdev, MS Clinical Research Pvt Ltd, Bangalore, Karnataka, India

Medical Director

Ritambhara K. R., MS Clinical Research Pvt Ltd, Bangalore, Karnataka, India

Clinical Research


Shin, Jung-Won. Molecular mechanisms of dermal aging and antiaging approaches. International journal of molecular sciences. 2019;20(9):2126.

Kohl E. Skin ageing. Journal of the European academy of dermatology and venereology. 2011;25(8):873-84.

Rinnerthaler, Mark. Oxidative stress in aging human skin. Biomolecules. 2015;5(2):545-89.

Baumann L. Skin ageing and its treatment. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland. 2007;211(2):241-51.

Paus R, Lübeck. How best to halt and/or revert UV‐induced skin ageing: strategies, facts and fiction. Experimental dermatology. 2008;17(3):228-9.

Siddharth M. Retinoids in the treatment of skin aging: an overview of clinical efficacy and safety. Clinical interventions in aging. 2006;1(4):327.

Humbert. Philippe G. Topical ascorbic acid on photoaged skin. Clinical, topographical and ultrastructural evaluation: double‐blind study vs. placebo." Experimental dermatology. 2003;12(3):237-44.

Murad H, Tabibian MP. The effect of an oral supplement containing glucosamine, amino acids, minerals, and antioxidants on cutaneous aging: a preliminary study. Journal of dermatological treatment. 2001;12(1):47-51.

Greul, Anne-Katrin. Photoprotection of UV-irradiated human skin: an antioxidative combination of vitamins E and C, carotenoids, selenium and proanthocyanidins. Skin Pharmacology and Physiology. 2000;15(5):307-15.

Ganceviciene R. Skin anti-aging strategies. Dermatoendocrinol. 2012;4:308-19.

Hunt, Katherine J, Hung SK, Ernst E. Botanical extracts as anti-aging preparations for the skin. Drugs & aging. 2010;27(12):973-85.

Stallings, Alison F, Lupo MP. Practical uses of botanicals in skin care." The Journal of clinical and aesthetic dermatology. 2009;2(1):36.

Thornfeldt C. Cosmeceuticals containing herbs: fact, fiction, and future. Dermatol Surg. 2005;31(7 Pt 2):873-80.

Soyun C. Differential effects of low-dose and high-dose beta-carotene supplementation on the signs of photoaging and type I procollagen gene expression in human skin in vivo. Dermatology. 2010;221(2):160-71.

Shalini S. Formulation and evaluation of carrot seed oil-based cosmetic emulsions. Journal of Cosmetic and Laser Therapy. 2009;21(2):99-107.

Kamrun N. Cardioprotective effect of Amaranthus tricolor extract in isoprenaline induced myocardial damage in ovariectomized rats. Biomedicine & Pharmacotherapy. 2018;103:1154-62.

Rownak J. Ethnopharmacological significance of Eclipta alba (L.) hassk. (Asteraceae). International Scholarly Research Notices. 2014;2014.

Mushtaq AD. Effect of Emblica officinalis (fruit) against UVB-induced photo-aging in human skin fibroblasts. Journal of ethnopharmacology. 2010;132(1):109-14.

Taro U. Oral intake of lingonberry and amla fruit extract improves skin conditions in healthy female subjects: A randomized, double‐blind, placebo‐controlled clinical trial." Biotechnology and applied biochemistry. 2019;66(5):870-9.

Vaughn, Alexandra R, Branum A, Sivamani RK. Effects of turmeric (Curcuma longa) on skin health: a systematic review of the clinical evidence. Phytotherapy Research. 2016;30(8):1243-64.

Laura V. Potential of curcumin in skin disorders. Nutrients. 2019;11(9):2169.

Bianca M. New insights in vitiligo treatments using bioactive compounds from Piper nigrum. Experimental and therapeutic medicine. 2019;17(2):1039-44.

Bahare S. Piper species: A comprehensive review on their phytochemistry, biological activities and applications. Molecules. 2019;24(7):1364.

Daswani, Poonam G, Gholkar MS, Birdi TJ. Psidium guajava: A single plant for multiple health problems of rural Indian population. Pharmacognosy reviews. 2017;11(22):167.

Díaz-de-Cerio, Elixabet. Health effects of Psidium guajava L. Leaves: An overview of the last decade. International journal of molecular sciences. 2017;18(4):897.

Germán M. Diseño y evaluación in vivo de fórmulas para acné basadas en aceites esenciales de naranja (Citrus sinensis), albahaca (Ocimum basilicum L) y ácido acético. Biomédica.2012;32(1):125-33.

Negar J, Cohen MM. The clinical efficacy and safety of Tulsi in humans: a systematic review of the literature. Evidence-Based Complementary and Alternative Medicine. 2017;2017.

Rahman U. Nutritional and therapeutic perspectives of Chia (Salvia hispanica L.): a review. Journal of food science and technology. 2016;53(4):1750-8.

Jeong, Kyoo S. Effectiveness of topical chia seed oil on pruritus of end-stage renal disease (ESRD) patients and healthy volunteers. Annals of Dermatology. 2010;22(2):143-8.

Hamdollah D. A review study on phytochemistry and pharmacology applications of Juglans regia plant. Pharmacognosy reviews. 2017;11(22):145.

Marina C, Chowienczyk P. Molecular mechanisms of arterial stiffening. Pulse. 2011;4(1):43-8.

Caligiuri, Stephanie PB. Elevated levels of pro-inflammatory oxylipins in older subjects are normalized by flaxseed consumption. Experimental gerontology. 52014;9:51-7.

Negar F. Prospective randomized controlled pilot study on the effects of almond consumption on skin lipids and wrinkles. Phytotherapy Research. 2019;33(12):3212-7.

Manshu J. Curry leaf (Murraya koenigii): A spice with medicinal property. MOJ Biol Med. 2017;2(3):00050.

Trisha D, Rao AR, Yadava PK. Chemomodulatory action of curry leaf (Murraya koenigii) extract on hepatic and extrahepatic xenobiotic metabolising enzymes, antioxidant levels, lipid peroxidation, skin and forestomach papillomagenesis. Nutrition research. 2003;23(1):1427-46.

Petar C. Glycerolic licorice extracts as active cosmeceutical ingredients: Extraction optimization, chemical characterization, and biological activity. Antioxidants. 2019;8(10):445.

Maedeh G. Efficacy of topical cinnamon gel for the treatment of facial acne vulgaris: A preliminary study. Biomedical Research and Therapy. 2019;6(1)2958-65.

Eid, Ahmad M. A Review on the Cosmeceutical and External Applications of Nigella sativa. Journal of tropical medicine. 2017;2017.

Aljabre, Salih HM, Omar M. Alakloby, and Mohammad A. Randhawa. Dermatological effects of Nigella sativa. Journal of dermatology & dermatologic surgery. 2015;19(2):92-8.

Yadav, Umesh CS, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharmaceutical biology. 2014;52(2):243-54.

Mio H. Double-blind study on effects of glucosyl ceramide in beet extract on skin elasticity and fibronectin production in human dermal fibroblasts. Anti-Aging Medicine. 2010;7(11):129-42.

Park, Hye Min H. Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation. International journal of dermatology. 2010;49(3):276-82.

Miguel, Maria G, Maria A. Neves, and Maria D. Antunes. Pomegranate (Punica granatum L.): A medicinal plant with myriad biological properties-A short review." Journal of Medicinal Plants Research. 2010;4(25):2836-47.

Hunt, Katherine J, Hung SK, Ernst E. Botanical extracts as anti-aging preparations for the skin. Drugs & aging. 2010;27(12):973-85.

Ng TB, Liu F, Wang ZT. Antioxidative activity of natural products from plants." Life sciences. 2000;66(8):709-23.

Ivana B. Skin ageing: natural weapons and strategies. Evidence-Based Complementary and Alternative Medicine. 2013;2013.

Matsui, Mary S. Non-sunscreen photoprotection: antioxidants add value to a sunscreen. Journal of Investigative Dermatology Symposium Proceedings. 2009;14(1).

Edzard E. First, do no harm’with complementary and alternative medicine. Trends in Pharma Sci. 2007;28(2):48-50.






Original Research Articles